Table 3

Relative gene expression profile of vitamin D receptor, 1 alpha-hydroxylase enzyme, and chemokine regulated on activation normal T-cell expressed and secreted and serum levels of calcium, phosphorus, magnesium, and parathyroid hormone in Holstein dairy cows during transition period

VDR1 α-OHaseRANTESCalcium
(mmol/L)
Phosphorous
(mmol/L)
Magnesium
(mmol/L)
PTH
(pg/mL)
3 weeks prior EDD2.51±0.54 a 0.034±0.01 a 0.058±0.15 a 2.04±0.11 a 2.39±0.10 a 1.18±0.31 a 16.07±2.01 a
At the day of parturition0.19±0.11b 1.71±0.76 b 0.016±0.04a 1.87±0.32b 2.61±0.12b 0.79±0.24a 27.20±7.99 b
3 weeks post-partum7.29±1.59c 0.05±0.034 a 0.001±0.003 a 2.50±0.12c 1.61±0.25c 0.92±0.20 a 17.35±2.67a

Wilks' Lambda test for time

p=0.001

p=0.001

p=0.476

p=0.013

p=0.001

p=0.232

p=0.005
  • a,b,c: Variables with different superscript letter in the same column are significantly different at p < 0.05.

  • EDD, expected date of delivery; 1α-OHase, 1 alpha-hydroxylase enzyme ; PTH, parathyroid hormone; RANTES, regulated on activation normal T-cell expressed and secreted ; VDR, vitamin D receptor.