Table 1

Quantitative real-time PCR primer sequences for evaluation of genetic expression of vitamin D receptor, 1 alpha-hydroxylase enzyme, and chemokine regulated on activation normal T-cell expressed and secreted during transition period in Holstein dairy cows

GeneAccession No #StrandPrimer sequence 5'−3'Reference
VDRNM_001167932FAGCCACCGGCTTCCATTTCANelson and others20
RAACAGCGCCTTCCGCTTCAT
1α-OHaseXM_588481FTGGGACCAGATGTTTGCATTCGCAalberts and others21
RTCTCAGACTGGTTCCTCATGGCT
RANTESNM_175827FCACCCACGTCCAGGAGTATTAalberts and others21
RCTCGCACCCACTTCTTCTCT
β-actinNM_173979.3FGGCATCCTGACCCTCAAGTANelson and others20
RCACACGGAGCTCGTTGTAGA
  • 1α-OHase, 1 alpha-hydroxylase enzyme; RANTES, regulated on activation normal T-cell expressed and secreted; VDR, vitamin D receptor.