TABLE 1:

Methods of activation evaluated to establish a protocol for sea turtle thromboelastography

ProtocolCup contents for each method of activation
1Kaolin (Kaolin, Haemoscope, Niles, IL.): 1-ml fresh whole blood is added to a kaolin vial. 20 µl of CaCl2 and 340 µl of kaolin-activated fresh whole blood.
2RapidTEG (RapidTEG, Haemoscope): 20 µl of CaCl2, 10 µl of reagent and 340 µl of citrated plasma.
3Fresh reptile thromboplastin (river cooter/box turtle): 20 µl of CaCl2, 20 µl of reagent and 330 µl of citrated plasma.
4Frozen reptile thromboplastin (sea turtle): 20 µl of CaCl2, 20 µl of reagent and 330 µl of citrated plasma.
5Dade Innovin Tissue Factor at 1:100 (Innovin, Siemens AG): 20 µl of CaCl2,36 µl reagent and 304 µl citrated plasma.
6Reptilase (Pefakit Reptilase, Pentapharm, Switzerland): 20 µl of CaCl2, 10 µl of reagent and 340 µl of citrated plasma.
7Rabbit thromboplastin (Sigma-Aldrich): 20 µl of CaCl2, 20 µl of reagent and 330 µl of citrated plasma.
8Fresh reptile thromboplastin (river cooter/box turtle): 20 µl of CaCl2, 10 µl of reagent and 340 µl of citrated plasma.
9Frozen reptile thromboplastin (sea turtle): 20 µl of CaCl2, 10 µl of reagent and 340 µl of citrated plasma.