Thromboelastography results from nine different methods of activation using pooled sea turtle citrated plasma, with the exception of protocol 1 which used whole blood

ProtocolR (minutes)K (minutes)α angle (°)MA (mm)LY30 (%)
1Kaolin (whole blood)98.3
2RapidTEG Reptilase96.6
3Fresh turtle thromboplastin 20 µl1.80.87237.50
4Frozen turtle thromboplastin 20 µl0.80.882.733.70
5Dade Innovin Tissue Factor 10090.9
7Rabbit thromboplastin86.6
8Fresh turtle thromboplastin 10 µl8.86.322.129.80
9Frozen turtle thromboplastin 10 µl2.30.878.035.50.1
  • Reagents 1, 2, 5 and 7 did not initiate any reaction after a minimum of 85 minutes. Reagent 6 produced a suboptimal tracing. Protocols 3 and 4 and modified versions using a reduced volume of thromboplastin, protocols 8 and 9, were considered successful protocols. Method 9 was selected as the optimal protocol based on measurable results consistent with mammalian methodology. Values are reported as the mean of the two samples performed apart from method 9, where 5 TEGs were performed.

  • α angle, clot formation rate; K, clot formation time; LY30, percentage of clot lysed after 30 minutes; MA, maximum amplitude (clot strength); n/a, not available; R, reaction time; TEG, thromboelastography.