TABLE 2:

Thromboelastography results from nine different methods of activation using pooled sea turtle citrated plasma, with the exception of protocol 1 which used whole blood

ProtocolR (minutes)K (minutes)α angle (°)MA (mm)LY30 (%)
1Kaolin (whole blood)98.3
2RapidTEG Reptilase96.6
3Fresh turtle thromboplastin 20 µl1.80.87237.50
4Frozen turtle thromboplastin 20 µl0.80.882.733.70
5Dade Innovin Tissue Factor 10090.9
6Reptilase1.1n/a49.518.40
7Rabbit thromboplastin86.6
8Fresh turtle thromboplastin 10 µl8.86.322.129.80
9Frozen turtle thromboplastin 10 µl2.30.878.035.50.1
  • Reagents 1, 2, 5 and 7 did not initiate any reaction after a minimum of 85 minutes. Reagent 6 produced a suboptimal tracing. Protocols 3 and 4 and modified versions using a reduced volume of thromboplastin, protocols 8 and 9, were considered successful protocols. Method 9 was selected as the optimal protocol based on measurable results consistent with mammalian methodology. Values are reported as the mean of the two samples performed apart from method 9, where 5 TEGs were performed.

  • α angle, clot formation rate; K, clot formation time; LY30, percentage of clot lysed after 30 minutes; MA, maximum amplitude (clot strength); n/a, not available; R, reaction time; TEG, thromboelastography.