Table 1:

Antibody characteristics and experimental procedures for immunohistochemistry

AntibodyManufacturerTypeCloneAntigen retrievalDilutionWash bufferIncubation
K19NovocastraMouse mAbb170Proteinase K1:100TBSO/N 4°C
PDGFRαAbcamRabbit AbPolyclonalTE buffer1:100PBSO/N 4°C
CD29BD BiosciencesMouse mAb18/CD29Citrate1:100PBSO/N 4°C
LamininAbcamRabbit AbPolyclonalProteinase K1:100PBSO/N 4°C
Bmi-1MilliporeMouse mAbF6TE buffer1:150PBSO/N 4°C
Glypican-3BiomosaicsMouse mAB1G12Citrate1:100PBSO/N 4°C
NF2SigmaRabbit AbPolyclonalProteinase K1:300PBS60 min. RT
MAC387AbcamMouse mABMAC387Proteinase K1:1,000PBSO/N 4°C
CD133eBioscienceRat mAB13A4Pepsin1:25PBSO/N 4°C
Isotype controlVector laboratoriesMouse IgGI-2000TE bufferAdjusted to concentrationPBSO/N 4°C
Isotype controlVector laboratoriesRabbit IgGI-1000TE bufferAdjusted to concentrationPBSO/N 4°C
  • Bmi-1, B-cell-specific Moloney murine leukaemia virus integration site 1;  K19, keratin 19; mAb, monoclonal antibody; MAC387, macrophage antigen 387; NF2, neurofibromatosis type 2; O/N, overnight; PDGFRα, platelet-derived growth factor receptor alpha polypeptide; RT, room temperature; TBS, Tris-buffered saline; TE, Tris/EDTA buffer.